Journal: Microbial Cell Factories

Article Title: Type 2 diabetes induced microbiome dysbiosis is associated with therapy resistance in pancreatic adenocarcinoma

doi: 10.1186/s12934-020-01330-3

Figure Lengend Snippet: T2D contributes to therapy resistance in PDAC. Schematic diagram of the experimental setup. Arrows indicate Streptozotocin (STZ) administration and fecal matter collection. C1, C2, C3, C4 refer to control groups that do not receive STZ or high fat diet D1, D2, D3, D4 refer to animals receiving STZ and put on high fat diet. C4 and D4 groups were further split into C4′ and D4′ to indicate groups that received Gem/Pac therapy ( a ). Blood glucose ( b ) and blood cholesterol ( c ) was measured at the end of 1 month to validate hyperglycemia and T2D onset in animals. After tumor implantation and 1 month of Gem/Pac, T2D group animals did not show any significant change in tumor volume ( e ) or weight ( f ), while the control tumors responded to the standard of care. *Indicates p value = 0.05

Article Snippet: Measurement of total cholesterol from mice serum were performed using cholesterol assay kit (Abcam) according to the manufacturer’s instructions.

Techniques: Control, Tumor Implantation

Journal: medRxiv

Article Title: SARS-CoV-2 disinfection in aqueous solution by UV 222 from a krypton chlorine excilamp

doi: 10.1101/2021.02.19.21252101

Figure Lengend Snippet: (A) The raw spectral emission from 200 - 300 nm of the filtered KrCl excilamp (USHIO Care222 ® ) was interpolated and relativized to the peak emission at 222 nm for use in UV dose calculations. (B) The absorbance spectrum from 200 - 300 nm of SARS-CoV-2 at ∼10 5 PFU/mL in cDMEM was measured for each of three biologically independent Tests for use in UV dose calculations. Expanded emission and absorbance spectra from 200 - 800 nm are shown in .

Article Snippet: The concentration of N protein in outgrowth assay supernatants was determined using the SARS-CoV-2 Antigen Quantitative Assay Kit (ELISA) method (ADS Biotec).

Techniques:

Journal: medRxiv

Article Title: SARS-CoV-2 disinfection in aqueous solution by UV 222 from a krypton chlorine excilamp

doi: 10.1101/2021.02.19.21252101

Figure Lengend Snippet: (A) SARS-CoV-2 titers measured by plaque assay 3 days after sample exposure to each UV 222 dose (dark circles) were fit with an exponential model starting at the mean initial (0 mJ/cm ) viral titer of 6.51×10 4 PFU/mL through responses up to and including 8 mJ/cm where PFU/mL first dropped below the assay detection limit (DL) of 2 PFU/mL (hollow circles). Error bars represent standard deviation of at least two technical replicates. (B) SARS-CoV-2 log 10 reductions (LR) of viral titers after exposure to each UV 222 dose (dark circles) were fit with a linear model forced through the origin at 0 mJ/cm through responses up to and including 8 mJ/cm where LR first exceeded the DL of 4.51 logs (hollow circles).

Article Snippet: The concentration of N protein in outgrowth assay supernatants was determined using the SARS-CoV-2 Antigen Quantitative Assay Kit (ELISA) method (ADS Biotec).

Techniques: Plaque Assay, Standard Deviation

Journal: medRxiv

Article Title: SARS-CoV-2 disinfection in aqueous solution by UV 222 from a krypton chlorine excilamp

doi: 10.1101/2021.02.19.21252101

Figure Lengend Snippet: (A) SARS-CoV-2 N gene damage immediately after UV treatment (Day 0) and after incubation of samples with host cells (Day 3) expressed as log 10 reduction of N1 (short amplicon) copies/µL in qPCR reactions. (B) SARS-CoV-2 N gene damage immediately after UV treatment (Day 0) expressed as log 10 reduction of N1-2 (long amplicon) copies/µL in qPCR reactions. (C) SARS-CoV-2 N protein concentration measured by ELISA expressed as log 10 reduction of N protein concentration (pg/mL) in samples immediately after UV treatment (Day 0) and after incubation of samples with host cells (Day 3). SARS-CoV-2 log 10 reductions of the N1 amplicon, N1-2 amplicon, or N protein versus UV 222 dose were fit with a linear model forced through the origin at 0 mJ/cm through responses up to and including 20 mJ/cm indicated by filled circles. Points not included in models are indicated by hollow circles.

Article Snippet: The concentration of N protein in outgrowth assay supernatants was determined using the SARS-CoV-2 Antigen Quantitative Assay Kit (ELISA) method (ADS Biotec).

Techniques: Incubation, Amplification, Protein Concentration, Enzyme-linked Immunosorbent Assay

Journal: medRxiv

Article Title: SARS-CoV-2 disinfection in aqueous solution by UV 222 from a krypton chlorine excilamp

doi: 10.1101/2021.02.19.21252101

Figure Lengend Snippet: (A) The raw spectral emission from 200 - 300 nm of the filtered excilamp (USHIO Care222 ® ) was interpolated and relativized to the peak emission at 222 nm for use in UV dose calculations and plotted on log scale to show orders of magnitude less but non-zero emission at filtered wavelengths > 240 nm. (B) The absorbance spectrum from 200 - 300 nm of SARS-CoV-2 at ∼10 5 PFU/mL in cDMEM was measured for each of three Tests for use in UV dose calculations.

Article Snippet: The concentration of N protein in outgrowth assay supernatants was determined using the SARS-CoV-2 Antigen Quantitative Assay Kit (ELISA) method (ADS Biotec).

Techniques: